ISSN 2756-3693
African Journal of Biology Vol. 1 (2), pp. 079-080, November, 2013. © International Scholars Journals
Short Communication
Characterization of potential ethylene-producing rhizosphere bacteria of Striga -infested maize and sorghum
Olubukola O. Babalola1,2*, Elie O. Osir2 and Abiodun I. Sanni1
1Department of Botany and Microbiology, University of Ibadan, Ibadan Nigeria 2International Centre of Insect Physiology and Ecology, Nairobi, Kenya
*Corresponding author; tel: 234-803-703-5965; e-mail: olubukola_babalola@hotmail.com
Accepted 12 November 2013
Abstract
Three rhizosphere bacteria, Pseudomonas sp., Enterobacter sakazakii and Klebsiella oxytoca, were analyzed for genetic variation. DNA fingerprint patterns of the three bacteria were markedly different when amplified with different primers. In total, 68 bands were produced by the three primers, 62 of which where variable. The number of polymorphic RAPD loci per isolate ranged from one to 13. Cluster analysis indicated that E. sakazakii and K. oxytoca are the most closely related of the three.
Keywords: Bacteria, RAPD-PCR, Striga hermonthica.