ISSN 2375-1134
African Journal of Agriculture ISSN 2375-1134 Vol. 11 (7), pp. 001-003, July, 2024. Available online at https://internationalscholarsjournals.org/journal/ajaoas/table-of-contents © International Scholars Journals
Full Length Research Paper
Advancements in Tissue Culture Methods for Stevia rebaudiana Bert Cultivation in Bangladesh
Mohammed Salim Uddin1, Mohammad Shaheed Hossain Chowdhury1, M. Muoztaba Mahfuzul Haque Khan1, Mohammad Belal Uddin1, Romel Ahmed1, Md. Azizul Baten2*
1Department of Forestry, Shah Jalal University of Science and Technology, Sylhet-3114, Bangladesh.
2Department of Statistics, Shah Jalal University of Science and Technology, Sylhet-3114, Bangladesh.
Accepted 28 March, 2024
Abstract
An experiment was conducted on in vitro culture of Stevia rebaudiana Bert, an important non-caloric sweetening herb to explore its potential for micro-propagation. Leaf, nodal and inter-nodal segments of the selected herb as explant were cultured on MS medium containing 2,4- D at 2, 3, 4 and 5 mg/L for callus induction. Inter-nodal segments initiated callus earlier than node and leaf. The highest amount of callus was found in MS medium with 3.0 mg/L 2,4-D and MS medium with 5.0 mg/L 2,4-D gave the poorest callus.
Key words: In vitro propagation, 2,4-D, Stevia rebaudiana.