ISSN 2375-1096
International Journal of Agroforestry and Silviculture ISSN 2375-1096 Vol. 7 (1), pp. 001-005, January, 2019. © International Scholars Journals
Full Length Research Paper
Isolation of high quality DNA and RNA from cambium of the East African Greenheart (Warburgia ugandensis)
E. Muge1*, K. Burg2, C. Kadu1, A. Muchugi1, S. Lemurt1 and R. Jamnadass1
1World Agro forestry Centre (ICRAF), Box 30677-00100, Nairobi, Kenya.
2Austrian Research Centers GmbH - ARC Department of Bioresources, A-2444 Seibersdorf, Austria.
Accepted 14 December, 2018
Abstract
The genus Warburgia belongs to the Canellaceae, a small family of tropical aromatic tree species important for their antibacterial and antifungal medicinal qualities amongst many other functions. Many published protocols for nucleic acid isolation failed to yield sufficiently good quality amounts for analysis. We have therefore developed a simple and fast CTAB (cetyl-trimethyl ammonium bromide)-based extraction protocol for routine isolation of high-quality nucleic acids. Good quality genomic DNA (gDNA) and RNA was obtained and was suitable for PCR amplification, restriction endonuclease digestion, Southern blotting and RT-PCR, respectively.
Key words: Nucleic acid extraction, polyphenols, polysaccharides, medicinal plants.