ISSN 2756-3340
African Journal of Infectious Diseases Research ISSN 4729-6836 Vol. 6 (5), pp. 001-006, May, 2019. © International Scholars Journals
Full Length Research Paper
Expression of chitinase and chitin binding proteins (CBP’s) by Listeria monocytogenes J0161 in biofilm and Co-culture broths
Prem Saran Tirumalai and Soam Prakash*
Environmental and Advanced Parasitology Lab, Department of Zoology, Faculty of Science,
Dayalbagh Educational Institute (Deemed University), Dayalbagh, Agra – 282110, India.
Accepted 17 October, 2018
Abstract
Listeria monocytogenes is a dreaded pathogen colonizes to form biofilm in the natural environments and in food processing facility. Biofilm state makes the bacterium resistant to antimicrobial agents. L. monocytogenes, an intracellular parasite also colonizes the mammalian epithelial cells in the gut. Chitinase and other chitinolytic proteins expressed by L. monocytogenes contribute to the colonization of the bacterium in the intestine. It has been reported that chitinase and chitin binding proteins are essential for attachment of the bacterium to the epithelial cells. In this study, we have pursued to analyze, if chitinase and chitin binding proteins play a role in biofilm formation and the expression of these proteins otherwise in a co-culture state. We report the expression pattern of these proteins, which supports our claim that chitinase and chitin binding proteins have a role to play in biofilm formation.
Key words: Listeria monocytogenes, chitinase, microarray, biofilm, co-culture, gene expression.