ISSN 2167-0447
International Journal of Agricultural Sciences ISSN: 2167-0447 Vol. 16 (4), pp. 001-006, April, 2026. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Efficient In Vitro Plantlet Regeneration of Mantisia wengeri (Zingiberaceae) from Rhizome-Derived Callus
Sudipta S. Das Bhowmik1*, Suman Kumaria2 and Pramod Tandon2
1Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati-781039, Assam, India,
2Plant Biotechnology Laboratory, Centre for Advanced Studies in Botany, North Eastern Hill University, Shillong - 793 022, India
Accepted 28 October, 2025
Mantisia wengeri Fischer is a rare and endemic zingiber of North-East India which has been traditionally used for curing bone fracture and gastro-intestinal ailments. However due to the extreme rarity of the germplasm, studies related to genetic diversity, phytochemical properties etc. in this plant has not been feasible. The study describes the development of high efficiency regeneration protocol for obtaining large- scale diverse plantlets from rhizomatous callus tissues in Murashige and Skoog medium incorporated with various concentration of growth regulators N6-benzyladenine (BA), α- Napthalene acetic acid (NAA), 2, 4-dichlorophenoxyacetic acid (2,4-D) and sucrose at elevated concentrations. Bud forming capacity (BFC) to a maximum of 33.5±3.7 was recorded from the organogenic callus tissues of M. wengeri within 8 - 10 weeks in MS medium supplemented with a combination of 10µM of BA and 40µM NAA at 10% sucrose. The callogenic plantlets are been transferred to the field for studying morphological and genetic variations.
Key words: - Conservation, regeneration, genetic variation, rhizomatous, callus