ISSN 2169-3048
International Journal of Biochemistry and Biotechnology ISSN 2169-3048 Vol. 9 (2), pp. 001-005, February, 2020. © International Scholars Journals
Full Length Research Paper
Evaluation of different carbon sources for bacterial cellulose production
Sherif M.A.S. Keshk1* and Kazuhiko Sameshima2
1Ain-Shams University, Institute of Environmental Studies and Research, Basic Science Department, Abbassia, Cairo11566, EGYPT.
2Kochi University, Faculty of Agriculture, Department of Forest Science, Nankoku, Kochi 783-8502, JAPAN.
Accepted 22 March, 2019
Abstract
The production of bacterial cellulose (BC) using Gluconacetobacter xylinus (=Acetobacter xylinum) ATCC 10245 from three categories of carbon sources (Monosaccharide, disaccharide and alcohol) was examined. Glycerol gave the highest yield, followed by glucose, fructose, inositol and saccharose. The highest production efficiency of glycerol might be due to the rate of its consumption (45.4%), which is lower than that of glucose (97%) . These results showed that the pH is not the only factor that affects the efficiency of BC production. The rate of consumption of carbon sources in the medium might play another important factor. From X-ray diffractometer, the crystalinity index of bacteria cellulose produce in presence of glucose and fructose have the highest values than those from other carbon sources.
Key word: Bacterial cellulose, Gluconacetobacter xylinus, Hestrin-Schramm’s medium, crystallinity index.