ISSN 2375-1266
African Journal of Environmental and Waste Management ISSN 2375-1266 Vol. 11 (9), pp. 001-007, September, 2024. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Analysis of the Molecular Diversity of Planctomycete-like Bacteria in the Context of Inosine Fermentation and Wastewater Treatment Processes
Hao Zhang, Ying Xu, Yifeng Ding, Qingxiang Yang*, Siwei Ren, Xueling Li, Xuanyu Chen, Yuanyuan Xu and Hongxin Hao
College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
Accepted 11 September, 2020
Abstract
In this study, the diversity of planctomycete-like bacteria in an inosine fermentation and a municipal wastewater treatment plants was investigated by denaturing gradient gel electrophoresis (DGGE) of nested polymerase chain reaction (nested PCR) amplified 16S rRNA gene fragments using planctomycete-specific forward primer and a bacteria universal reverse primer. The bacterial community structures in the two wastewater treatment systems were obviously different due to the different treatment processes and different wastewater characteristics. The highest similarity of the microbial community structure happened in the two samples from the acidification and the anaerobic tank in inosine fermentation plant reaching 67%. While the two samples from the anaerobic and the aerobic tank of municipal wastewater treatment plant reached 66%. Ten sequences were recovered from the DGGE gel and were assigned to Clostridia, Proteobacteria, Bacteroidete and Candidate division based on the phylogenetic analysis. The two sequences grouped into Candidate division had more than 92% similarities with the Planctomycete-like bacterial clones. However, no anaerobic ammonium oxidation (anammox) activities were detected in the samples of these two wastewater treatment plants.
Key words: Planctomycete-like bacteria, wastewater treatment, denaturing gradient gel electrophoresis (DGGE).