ISSN 2756-326X
Advances in Agriculture and Agricultural Sciences ISSN 2756-326X Vol. 13 (3), pp. 001-006, March, 2025. Available online at www.internationalscholarsjournals.org © International Scholars Journals
Full Length Research Paper
Exploring Cryopreservation Methods for Strychnos pseudoquina A. St. Hil Seeds
Vanisse de Fátima Silva1, Juliana de Fátima Sales2, Fabiano Guimarães Silva2, Rafael Cândido Campos3, Adriene de Cássia Branquinho3 e Vânia Aparecida Silva4
1Bolsista DCR CNPq-FAPEG. Laboratório de Sementes – Instituto Federal de Educação, Ciência e Tecnologia Goiano
– Campus Rio Verde. CEP 75900-000. Rio Verde, GO
2Professor do IFGoiano – Campus Rio Verde. julianacefetrv@yahoo.com.br
3Bolsista de IC do CNPq
4Empresa de Pesquisa Agropecuária de Minas Gerais, Unidade Regional Epamig Sul de Minas. Caixa Postal 176, CEP
37200-000, Lavras- MG- Brasil
Accepted 16 January, 2024
Abstract
This study aimed to evaluate the effects of storage in liquid nitrogen at -196°C on Strychnos pseudoquina A. St. Hil. seeds with moisture contents of either 6 or 7%wb subjected to quick thawing (in a water bath at 37°C for 10 minutes) and slow thawing (at room temperature (26°C±2°C) for 2 hours) and then stored for 2, 4, and 6 months. The germination percentage in cryopreserved quina seeds decreased by approximately 12.4% compared to non-cryopreserved seeds (95 to 83.22%), regardless of the moisture content. In cryopreserved seeds with 7% moisture content, the germination percentage of seeds thawed quickly versus slowly differed only for those seeds stored for 2 months; the seeds subjected to fast thawing showed lower germination percentages than those subjected to slow thawing. Quina seed cryopreservation may be viable for seed conservation as germination remained above 80% in all samples.
Keywords: Storage, Germination, Viability.