Advances in Food Science and Technology

Advances in Food Science and Technology ISSN: 6732-4215 Vol. 8 (3), pp. 001-004, March, 2020. © International Scholars Journals

Full Length Research Paper

In vitro multiplication of Ocimum gratissimum L. through direct regeneration

C. Gopi*, Y. Nataraja Sekhar and P. Ponmurugan

Department of Biotechnology, K. S. Rangasamy College of Technology, Tiruchengode - 637 209, Namakkal District, Tamilnadu. INDIA.

Accepted 11 September, 2019

Abstract

The objective of this study was to develop a rapid system for regeneration of the important medicinal plant, Ocimum gratissimum L, from nodal explant. Single node explants were inoculated on basal MS (Murashige and Skoog, 1962) medium containing 3% (w/v) sucrose, supplemented with different concentrations and combinations of 6-benzylaminopurine (BAP), kinetin (KN), indole-3-acetic acid (IAA) or indole- 3-butyric acid (IBA) for direct plant regeneration. Maximum numbers of shoot (14.3±1.5) were observed on the medium containing 0.5 mg/l BAP and 0.25 mg/l IAA after four weeks of culture. Regenerated shoots were separated and rooted on same half strength MS medium supplemented with 0.5 mg/l of IAA alone for three weeks. Well-developed complete plantlets were transferred on to specially made plastic cup containing soilrite. Acclimatized plantlets were successfully grown in garden soil.

Key words: Ocimum gratissimum L., node explants, multiplication, direct regeneration, plantlets.