Advances in Food Science and Technology

Advances in Food Science and Technology ISSN: 6732-4215 Vol. 8 (3), pp. 001-008, March, 2020. © International Scholars Journals

Full Length Research Paper

Effect of myoinositol and zinc on sporangiospore-yeast transformation of MUCOR  CIRCINELLOIDES  Tieghe

C. O. Omoifo

Department of Crop Science, Ambrose Alli University, Ekpoma, Nigeria. E-mail: coomoifo@yahoo.com.

Accepted 14 September, 2019

Abstract

Environmental factors influence mould-yeast interconversion. Such factors may trigger changes at the cellular level. Yeastlike cells induced from sporangiospores of MUCOR species consist of central globose mothercell, which produce multiple buds by blastic action. This study examines the effect of synthetic broth on sporangiospores of M. CIRCINELLOIDES. Sporangiospores of M. CIRCINELLOIDES converted to determinate thallic subtypes including enterothallic- holothallic- and holoblastic conidia, as well as terminal budding yeast cells in synthetic broth treatments with zinc and myoinositol (myo) supplementations. In a study monitored at 24 h intervals, profiling gave 2-phase expression in some treatments and this was influenced by the presence of thallic forms. However, sigmoid pattern was obtained in control broth as well as in 0.25 mM Zn²⁺ and 1.0 – 3.0 mM myo treatments. Such treatments enhanced growth by 32.95, 65.07 and 63.82%, respectively, over control mean growth. Proliferating yeast cells induced from sporangiospores of M. CIRCINELLOIDES transformed through several transient morphologies in a sequential manner, protoplast being the cross over form. Zinc and myo supplementation enhanced yeast induction. It was strongly suggested that they acted as second messengers in signal transduction and consequent yeast induction. Whereas thallic subtypes were present, subsequent transformation of their cytosolic contents, following thallic cell wall rupture, followed the same pattern of lateral morphogenetic transformation.

Key words: Mucor circinelloides, sporangiospores, synthetic broth, zinc, myoinositol, induced morphology.